5J48
PKG I's Carboyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with 8-pCPT-cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97741 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.510, 59.240, 67.320 |
Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
Resolution | 34.952 - 1.490 |
R-factor | 0.1646 |
Rwork | 0.163 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ku7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.253 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.240 |
High resolution limit [Å] | 1.490 |
Rmerge | 0.047 |
Number of reflections | 44551 |
<I/σ(I)> | 14.1 |
Completeness [%] | 99.1 |
Redundancy | 4.2 |
CC(1/2) | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 400, 200 mM CaAc, 0.1M NaAc pH 4.5-4.8 |