5J0I
De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 44.158, 44.158, 153.326 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.330 - 2.202 |
R-factor | 0.2279 |
Rwork | 0.225 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.025 |
RMSD bond angle | 1.988 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1616) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.330 |
High resolution limit [Å] | 2.200 |
Number of reflections | 4361 |
<I/σ(I)> | 14.32 |
Completeness [%] | 86.7 |
Redundancy | 18.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.2 M Sodium Malonate pH 5.0 |