5IYP
Protruding domain of GII.4 human norovirus CHDC2094 in complex with HBGA type A (triglycan)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97625 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 89.710, 106.120, 135.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.248 - 1.270 |
R-factor | 0.1361 |
Rwork | 0.135 |
R-free | 0.15940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iyn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.246 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.250 | 1.280 | |
High resolution limit [Å] | 1.250 | 5.590 | 1.250 |
Rmerge | 0.057 | 0.026 | 0.687 |
Number of reflections | 175960 | ||
<I/σ(I)> | 12.8 | 48.26 | 1.69 |
Completeness [%] | 98.8 | 98.1 | 97.1 |
Redundancy | 3.9 | ||
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | Sodium citrate, PEG3350 |