5IWA
Crystal structure of the 30S ribosomal subunit from Thermus thermophilus in complex with the GE81112 peptide antibiotic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2010-08-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 413.140, 413.140, 173.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.034 - 3.500 |
| R-factor | 0.1687 |
| Rwork | 0.166 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zm6 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS (VERSION May 10, 2010) |
| Data scaling software | XSCALE (VERSION May 10, 2010) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 160.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.165 | 2.379 |
| Number of reflections | 187400 | |
| <I/σ(I)> | 9.79 | 1.46 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 11.3 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | HEPES-KOH, MgCl2, NH4Cl, beta-mercaptoethanol, MPD, spermidine |
