5IU6
Crystal structure of E.coli purine nucleoside phosphorylase with 7-deazahypoxanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.8 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 120.370, 120.370, 238.971 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.860 - 2.510 |
R-factor | 0.15701 |
Rwork | 0.155 |
R-free | 0.20047 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.428 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.870 | 2.650 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmerge | 0.065 | 0.170 |
Number of reflections | 35075 | |
<I/σ(I)> | 10.4375 | |
Completeness [%] | 98.1 | |
Redundancy | 4.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 294 | AMMONIUM SULPHATE |