5IOK
Crystal structure of Taf14 YEATS domain in complex with histone H3K9cr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-23 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.095, 113.095, 26.176 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.648 - 2.220 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d7e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.650 | 2.260 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 9584 | |
| <I/σ(I)> | 24.9 | 2.4 |
| Completeness [%] | 97.1 | 72.6 |
| Redundancy | 3.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 44% PEG600 (v/v) and 0.2 M citric acid (pH 6.0) |
