5IKJ

Structure of Clr2 bound to the Clr1 C-terminus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763,0.9792,0.9763,0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	103.499, 103.499, 135.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.325 - 2.300
R-factor	0.2152
Rwork	0.214
R-free	0.24310
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.523
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.325	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.042	0.845
Number of reflections	37719
<I/σ(I)>	24.9	2.2
Completeness [%]	100.0	100
Redundancy	7.6	7.9
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	291	0.1 M Tris-bicine 30 mM Sodium Fluoride 30 mM Sodium Bromide 30 mM Sodium Iodide 18% Ethylene glycol 9% PEG 8000