5IIQ
Structure of the SPX-TTM domain fragment of the yeast inorganic polyphophate polymerase Vtc4 (form B).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-22 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000020 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 92.041, 92.041, 301.732 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.880 - 3.030 |
R-factor | 0.2266 |
Rwork | 0.225 |
R-free | 0.26240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g3r |
RMSD bond length | 0.008 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.030 | 3.030 |
Number of reflections | 15492 | |
<I/σ(I)> | 28.4 | 2.99 |
Completeness [%] | 99.0 | 97.4 |
Redundancy | 20.7 | 18.3 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 1.5M AmSO4, 4% PEG 1000 |