5I8Q
S. cerevisiae Prp43 in complex with RNA and ADPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 168.063, 154.031, 78.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.446 - 4.200 |
R-factor | 0.2629 |
Rwork | 0.260 |
R-free | 0.28610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kx2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.567 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.340 | |
High resolution limit [Å] | 4.200 | 9.250 | 4.200 |
Rmerge | 0.255 | 0.038 | 1.054 |
Number of reflections | 15735 | ||
<I/σ(I)> | 8.67 | 34.24 | 2.08 |
Completeness [%] | 99.5 | 95.7 | 99.9 |
Redundancy | 6.05 | ||
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 292 | Crystallization buffer was prepared by mixing a solution containing 100 mM MES-NaOH, pH 6.0, 100 mM NaOAc, 8% PEG 10K and an additive solution (20% acetonitrile, 5% n-Dodecyl-?-D-maltoside) in a 5:1 (v:v).Microbatch drops were formed by mixing 1.8 ?l of the crystallization buffer with 1.2 ?l of the preformed complex |