5I8L
Crystal structure of the full-length cell wall-binding module of Cpl7 mutant R223A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.05793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.524, 50.331, 86.089 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.873 - 2.801 |
R-factor | 0.2088 |
Rwork | 0.206 |
R-free | 0.28910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cvd |
RMSD bond length | 0.005 |
RMSD bond angle | 1.269 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (dev_1965) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.040 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.075 | 0.150 |
Number of reflections | 2800 | |
<I/σ(I)> | 10.8 | 6.3 |
Completeness [%] | 81.2 | 90.8 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.1 M tri-Sodium citrate |