5HO4
Crystal structure of hnRNPA2B1 in complex with 10-mer RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-06 |
| Detector | RIGAKU |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.604, 38.959, 70.442 |
| Unit cell angles | 90.00, 116.26, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.18493 |
| Rwork | 0.183 |
| R-free | 0.22005 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.961 |
| Data reduction software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.170 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 21311 |
| <I/σ(I)> | 18.4 |
| Completeness [%] | 92.4 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Tri-Sodium citrate, 20% (w/v) polyethylene glycol 3,350 |
