5HKV
The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with lincomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.971 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 280.885, 280.885, 873.522 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.660 |
| R-factor | 0.1896 |
| Rwork | 0.187 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wce |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.438 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.640 | |
| Rmerge | 0.237 | 0.972 |
| Number of reflections | 223602 | |
| <I/σ(I)> | 9.08 | 1.37 |
| Completeness [%] | 98.0 | 78.1 |
| Redundancy | 10 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 1:2 MPD: EtOH, Hepes pH=7.6 10mM, Mg2Cl 10mM, KCl 60mM, NH4Cl 15mM |
