5HCQ

Crystal structure of antimicrobial peptide Oncocin d15-19 bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	210.053, 450.752, 623.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.923 - 2.801
R-factor	0.2027
Rwork	0.200
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.222
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	49.923	2.970
High resolution limit [Å]	2.800	8.300	2.800
Rmerge	0.161	0.024	1.395
Rmeas	0.180	0.027	1.557
Total number of observations	7162802
Number of reflections	1426619	56278	227225
<I/σ(I)>	10.8	50.24	1.29
Completeness [%]	99.6	98.8	98.9
Redundancy	5.021
CC(1/2)		0.999	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME