5H3T
m7G cap bound to GEMIN5-WD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 113.159, 91.007, 153.717 |
Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
Resolution | 49.326 - 2.571 |
R-factor | 0.1738 |
Rwork | 0.171 |
R-free | 0.22810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ymu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.330 | 2.610 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.070 | 0.734 |
Number of reflections | 96204 | |
<I/σ(I)> | 16.4 | 2 |
Completeness [%] | 97.9 | 71.1 |
Redundancy | 7 | 5.5 |
CC(1/2) | 0.990 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1 M Bis-Tris propane, 0.2 M Na/K phosphate, 20% PEG 3350 |