5GT3
Crystal structure of nucleosome particle in the presence of human testis-specific histone variant, hTh2b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.887, 110.078, 182.811 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.700 - 2.910 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x1t |
RMSD bond length | 0.011 |
RMSD bond angle | 1.276 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.114 | 0.715 |
Number of reflections | 46654 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 96.8 | 95 |
Redundancy | 6.1 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 60-70mM KCl, 70-90mM MnCl2, 24% MPD |