5GT0
Crystal structure of nucleosome complex with human testis-specific histone variants, Th2a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.788, 108.966, 170.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.800 - 2.820 |
R-factor | 0.214 |
Rwork | 0.210 |
R-free | 0.28000 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.338 |
Data scaling software | HKL-2000 (Scala) |
Phasing software | CNX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.597 | |
Number of reflections | 45145 | |
<I/σ(I)> | 16.2 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.2 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 60-70mM KCl, 70-90mM MnCl2, 24% MPD |