5GMB
Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruiginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-03-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.796, 59.796, 124.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.580 - 1.620 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cnd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.030 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.060 | 0.730 |
| Number of reflections | 29132 | |
| <I/σ(I)> | 19 | 3.9 |
| Completeness [%] | 95.4 | 71.5 |
| Redundancy | 9.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | MgCl2, CaCl2, PEG MME 550, PEG 20000, MOPS/Na-HEPES pH7.5 |
