5GLJ

Crystal Structure of PDZ1 Domain of Human Protein Tyrosine Phosphatase PTP-Bas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2014-07-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	32.034, 57.571, 168.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.953 - 1.600
R-factor	0.1717
Rwork	0.170
R-free	0.20570
RMSD bond length	0.006
RMSD bond angle	1.083
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	42069
<I/σ(I)>	21.3
Completeness [%]	99.5
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M Magnesium chloride hexahydrate , 0.1 M BIS-TRIS pH5.5, 25 % w/v Polyethylene glycol 3350