5G26
Unveiling the Mechanism Behind the in-meso Crystallization of Membrane Proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-10 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 115.944, 119.925, 39.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.500 - 2.420 |
R-factor | 0.22448 |
Rwork | 0.222 |
R-free | 0.27844 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e1s |
RMSD bond length | 0.012 |
RMSD bond angle | 1.527 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.700 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.140 | 0.780 |
Number of reflections | 10728 | |
<I/σ(I)> | 12.3 | 2.9 |
Completeness [%] | 99.1 | 91.8 |
Redundancy | 10.2 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.3 | 100MM TRISODIUM CITRATE/CITRIC ACID PH 4.3, 113MM NACL, 78MM MGCL2, 27% PEG 400 (V/V); MONOPALMITOLEIN USED FOR IN-MESO CRYSTALLISATION |