5G1K
A triple mutant of DsbG engineered for denitrosylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.920, 57.220, 86.040 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 42.872 - 1.960 |
| R-factor | 0.1965 |
| Rwork | 0.192 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v57 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 40638 | |
| <I/σ(I)> | 15.58 | 3.53 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM CITRATE PH 3.75, 0.2 M AMMONIUM SULFATE, 20% PEG 4000 |
