5FJM
Structure of L-Amino acid deaminase from Proteus myxofaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-16 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.760, 93.710, 74.690 |
Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
Resolution | 46.850 - 2.000 |
R-factor | 0.17855 |
Rwork | 0.177 |
R-free | 0.20898 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gag |
RMSD bond length | 0.006 |
RMSD bond angle | 1.137 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.080 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.510 |
Number of reflections | 65982 | |
<I/σ(I)> | 12 | 2.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 15% PEG 6K, 0.1 M MES (PH 6.5), 10% MPD |