5F8V
Crystal structure of PLP bound phosphoserine aminotransferase (PSAT) from Trichomonas vaginalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-19 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.967, 235.434, 134.520 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 37.420 - 2.140 |
R-factor | 0.21022 |
Rwork | 0.209 |
R-free | 0.23905 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bjn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.253 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.440 | |
Number of reflections | 177429 | |
<I/σ(I)> | 7.9 | 3.3 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25-30% PEG 3350 (w/v),100mM sodium acetate pH 4.5-5.0, 200mM sodium malonate, 10% glycerol (v/v) |