5F7F
Human T-cell immunoglobulin and mucin domain protein 4 (hTIM-4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.485, 43.780, 44.726 |
| Unit cell angles | 77.32, 86.78, 85.34 |
Refinement procedure
| Resolution | 27.633 - 1.502 |
| R-factor | 0.1725 |
| Rwork | 0.172 |
| R-free | 0.18940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bi9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.057 | 0.283 |
| Number of reflections | 34370 | |
| <I/σ(I)> | 24.14 | 5.82 |
| Completeness [%] | 96.1 | 94.6 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M phosphate/citrate, 40%(v/v) PEG300 |
