5F1Q
Crystal Structure of Periplasmic Dipeptide Transport Protein from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97916 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 289.290, 59.745, 80.631 |
Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
Resolution | 28.311 - 1.956 |
R-factor | 0.1674 |
Rwork | 0.166 |
R-free | 0.20020 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.131 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.513 | |
Number of reflections | 93243 | |
<I/σ(I)> | 7.98 | 1.42 |
Completeness [%] | 98.4 | 98.3 |
Redundancy | 4.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.1 M phosphate-citrate, pH 4.2, 40:5(v/v) PEG 600 |