5EXZ
Crystal structure of purified recombinant CPV1 Polyhedra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.170, 103.170, 103.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.570 - 1.900 |
| R-factor | 0.152 |
| Rwork | 0.146 |
| R-free | 0.20360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BUSTER (2.10.1) |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.200 | 1.900 |
| Rmerge | 0.268 | 0.107 | |
| Rmeas | 0.322 | 0.125 | 0.688 |
| Rpim | 0.165 | 0.063 | 0.348 |
| Total number of observations | 42463 | ||
| Number of reflections | 13734 | ||
| <I/σ(I)> | 4.5 | 1.9 | |
| Completeness [%] | 94.5 | 92.2 | 93.4 |
| Redundancy | 3.3 | 3.4 | 3.4 |
| CC(1/2) | 0.982 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 300 | The crystals used to determine this structure were directly purified from cells, in vivo crystallization in the cytoplasm of the cell |
