5EX8
Structure of P450 StaF from glycopeptide antibiotic A47934 biosynthesis; ethylene glycol cryo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.050, 110.050, 93.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.670 - 2.100 |
R-factor | 0.19565 |
Rwork | 0.194 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o1a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.193 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.072 | 0.308 |
Number of reflections | 36757 | |
<I/σ(I)> | 19.8 | 4.2 |
Completeness [%] | 95.1 | 91.6 |
Redundancy | 6.2 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 1.2 M NH4PO4, 0.3 M K2HPO4, 0.1 M NH4PO4 citrate (pH 4.2) |