5EX2
Crystal structure of cyclophilin AquaCyp293 from Hirschia baltica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.940, 72.730, 73.930 |
| Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
| Resolution | 47.874 - 1.294 |
| R-factor | 0.1699 |
| Rwork | 0.169 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.874 | 1.330 |
| High resolution limit [Å] | 1.294 | 1.294 |
| Rmerge | 0.127 | 1.140 |
| Number of reflections | 122350 | |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 97.1 | 93.7 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 % PEG20000, 20 % PEGMME550 in 0.03 M CaCl2, 0.03M MgCl2, 0.1 M Mops/Hepes-Na pH 7.5 |
