5ERB
Ketosynthase from module 5 of the bacillaene synthase from Bacillus amyloliquefaciens FZB42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-02 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.758, 319.132, 103.470 |
| Unit cell angles | 90.00, 110.26, 90.00 |
Refinement procedure
| Resolution | 97.070 - 4.200 |
| R-factor | 0.29112 |
| Rwork | 0.288 |
| R-free | 0.34088 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.651 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 97.070 |
| High resolution limit [Å] | 4.200 |
| Number of reflections | 23712 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 79.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 1.75 uL protein solution (9.9 mg/mL BaeKS5, 150 mM NaCl, 15 mM HEPES, pH 7.5) with 0.5 uL crystallization buffer (1.1 M LiSO4, 0.1 M Tris, pH 8.0) |
