5EO9
Crystal Structure of the complex of Dpr6 Domain 1 bound to DIP-alpha Domain 1+2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2013-05-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 130.247, 49.299, 54.707 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.107 - 2.299 |
R-factor | 0.2047 |
Rwork | 0.202 |
R-free | 0.25190 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.683 |
Data reduction software | HKL-2000 (0.98.708) |
Data scaling software | HKL-2000 (0.98.708) |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.299 | 2.300 |
Rmerge | 0.082 | 0.684 |
Number of reflections | 16293 | |
<I/σ(I)> | 29.4 | 1.5 |
Completeness [%] | 99.8 | 96.9 |
Redundancy | 11.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 44% PEG400, 0.1 M HEPES pH 7, 0.2 M Li2SO4 |