5ELI
Triggering receptor expressed on myeloid cells 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 125.763, 125.763, 183.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.689 - 3.098 |
| R-factor | 0.2611 |
| Rwork | 0.261 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.098 | 3.098 |
| Rmerge | 0.109 | 0.800 |
| Number of reflections | 16285 | |
| <I/σ(I)> | 20.96 | 1.72 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.9 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 290 | 2 M NaCl, 0.2 M MgCl2, 0.2 M NDSB 201, 100 mM HEPES pH 7.1 |
