5EJJ
Crystal structure of UfSP from C.elegans
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.531, 149.808, 226.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.278 - 2.800 |
R-factor | 0.2066 |
Rwork | 0.206 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.301 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.071 | 0.421 |
Number of reflections | 66417 | |
<I/σ(I)> | 28.6 | 1.65 |
Completeness [%] | 93.8 | 77.3 |
Redundancy | 9.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Bicine, Ammonium phosphate |