5E3C
Structure of human DPP3 in complex with hemorphin like opioid peptide IVYPW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972390 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.669, 106.386, 65.121 |
Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
Resolution | 45.560 - 2.765 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.25280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t6b |
RMSD bond length | 0.003 |
RMSD bond angle | 0.507 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.560 | 2.860 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.211 | 0.502 |
Number of reflections | 20651 | |
<I/σ(I)> | 4.39 | 1.44 |
Completeness [%] | 97.9 | 84.87 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.056 Sodium phosphate monobasic monohydrate, 1.344M Potassium phosphate dibasic |