5E04
Crystal structure of Andes virus nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.526, 99.652, 136.388 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.017 - 2.250 |
R-factor | 0.2178 |
Rwork | 0.215 |
R-free | 0.25190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e06 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.211 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.250 |
Number of reflections | 33842 |
<I/σ(I)> | 23.2 |
Completeness [%] | 90.8 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100mM HEPES (pH 7.5), 200mM L-proline and 10%(w/v) PEG3350 |