5DYV
AbyU - wildtype
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2015-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9730 |
Spacegroup name | P 1 |
Unit cell lengths | 61.800, 73.590, 86.190 |
Unit cell angles | 112.87, 90.04, 89.98 |
Refinement procedure
Resolution | 79.410 - 2.500 |
R-factor | 0.19946 |
Rwork | 0.197 |
R-free | 0.25298 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.848 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.410 | |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.120 | 0.358 |
Number of reflections | 52922 | |
<I/σ(I)> | 6.4 | |
Completeness [%] | 92.4 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02 M magnesium chloride, 0.1 M HEPES pH 7.5 18% w/v poly(acrylic acid) sodium salt MW 5100 |