5DYE
CRYSTAL STRUCTURE OF THE FULL LENGTH S156E MUTANT OF HUMAN AQUAPORIN 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.873 |
Spacegroup name | P 3 1 2 |
Unit cell lengths | 174.286, 174.286, 100.858 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 150.760 - 3.500 |
R-factor | 0.28036 |
Rwork | 0.279 |
R-free | 0.31096 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.084 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.000 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.095 | 0.799 |
Number of reflections | 22191 | |
<I/σ(I)> | 13 | 1.9 |
Completeness [%] | 98.9 | 99.8 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 281 | 21% PEG 400, 0.1M Tris pH 7.9, 0.1 M NaCl, 3% 1,6 hexanediol and 4% 1,3-propanediol as additive to the drop only |