5DM3
Crystal Structure of Glutamine Synthetase from Chromohalobacter salexigens DSM 3043(Csal_0679, TARGET EFI-550015) with bound ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 132.983, 221.339, 199.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.064 - 2.600 |
R-factor | 0.2043 |
Rwork | 0.201 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lni |
RMSD bond length | 0.009 |
RMSD bond angle | 1.229 |
Data reduction software | DIALS |
Data scaling software | DIALS (0.5.12) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 199.350 | 199.350 | 2.640 |
High resolution limit [Å] | 2.600 | 14.240 | 2.600 |
Rmerge | 0.171 | 0.078 | 1.711 |
Rpim | 0.068 | 0.033 | 0.809 |
Total number of observations | 654205 | 3712 | 22554 |
Number of reflections | 90297 | ||
<I/σ(I)> | 7.1 | 14.6 | 0.8 |
Completeness [%] | 99.9 | 99 | 98.9 |
Redundancy | 7.2 | 6 | 5.1 |
CC(1/2) | 0.994 | 0.990 | 0.307 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Protein (5mM MgCl2, 5mM ATP, 10 mM HEPES pH 7.5, 5 mM DTT); Reservoir (.1 M Sodium Citrate:HCl pH 5.6, 10% (w/v) PEG 4000, 10% (v/v) 2-Propanol); Cryoprotection (20% Ethylene glycol, 80% Reservoir) |