5DKT
N-terminal His tagged apPOL exonuclease mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.775, 141.775, 148.759 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.346 - 2.900 |
| R-factor | 0.2682 |
| Rwork | 0.265 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kfn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.549 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.345 | 0.112 | |
| Rmeas | 0.339 | 0.125 | |
| Rpim | 0.195 | 0.055 | 0.668 |
| Total number of observations | 82305 | ||
| Number of reflections | 19706 | ||
| <I/σ(I)> | 2.5 | ||
| Completeness [%] | 97.8 | 96.7 | 96.4 |
| Redundancy | 4.2 | 4.8 | 3.4 |
| CC(1/2) | 0.990 | 0.018 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 30%(w/v) PEG monomethyl ether 5000 |
