5DFX
Structure of the parental state of GAF3 from Slr1393 of Synechocystis sp. PCC6803 (in vivo assembled protein/chromophore)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97916 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.120, 63.120, 118.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 1.800 |
R-factor | 0.16585 |
Rwork | 0.164 |
R-free | 0.19902 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 2.428 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.960 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 0.301 |
Number of reflections | 22947 | |
<I/σ(I)> | 19.19 | 5.42 |
Completeness [%] | 99.7 | 99 |
Redundancy | 6.63 | 6.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 | 0.02 M Tris pH 7.0, 0.1 M sodium chloride, 7.7 % (w/v) PEG 4000 |