5D3D
Crystal structure of Staphylococcal Superantigen-Like protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-03-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.000, 79.220, 96.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.200 - 1.940 |
| R-factor | 0.17874 |
| Rwork | 0.176 |
| R-free | 0.22431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SSL4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.206 |
| Data reduction software | MOSFLM (1.0.6) |
| Data scaling software | Aimless (1.7.6) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.200 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.093 | 0.730 |
| Number of reflections | 33556 | |
| <I/σ(I)> | 11.1 | 2.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | PEG 3350, potassium thiocyanate |
