5CYP
GTPase domain of Septin 9 in complex with GTP-gamma-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.587, 79.156, 108.217 |
Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
Resolution | 45.654 - 2.893 |
R-factor | 0.2544 |
Rwork | 0.252 |
R-free | 0.29740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.594 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 | |
High resolution limit [Å] | 2.890 | 8.550 | 2.890 |
Rmerge | 0.121 | 0.026 | 0.652 |
Rmeas | 0.145 | 0.032 | 0.789 |
Total number of observations | 85852 | ||
Number of reflections | 27269 | 1083 | 4266 |
<I/σ(I)> | 8.57 | 28.22 | 1.9 |
Completeness [%] | 97.6 | 95.1 | 95.2 |
Redundancy | 3.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | 20% PEG1500, 20% Glycerol |