5CTR
Crystal structure of human SART3 HAT-C domain-human USP4 DUSP-UBL domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 115.202, 115.202, 306.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.012 |
| R-factor | 0.31 |
| Rwork | 0.309 |
| R-free | 0.33370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ctq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.305 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.107 | 0.413 |
| Number of reflections | 41894 | |
| <I/σ(I)> | 11.7 | 2.26 |
| Completeness [%] | 98.0 | 96.2 |
| Redundancy | 4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES pH 6.8, 200 mM magnesium chloride, 15% PEG 4000 |
