5CR2
E. coli MazF in complex with single strand DNA substrate analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.980 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.960, 113.960, 47.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.808 - 2.900 |
R-factor | 0.234 |
Rwork | 0.223 |
R-free | 0.29010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mdx |
RMSD bond length | 0.003 |
RMSD bond angle | 0.730 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.801 | 3.170 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 7461 | |
<I/σ(I)> | 8.96 | 2.25 |
Completeness [%] | 99.4 | 96.7 |
Redundancy | 8.95 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.17 M Sodium acetate trihydrate, 0.085 M Tris hydrocloride pH 8.5, 25.5% w/v PEG4000, 15% Glycerol |