5CKK
Crystal structure of 9DB1* deoxyribozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.155, 83.155, 55.995 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.446 - 2.801 |
R-factor | 0.2755 |
Rwork | 0.275 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cki |
RMSD bond length | 0.015 |
RMSD bond angle | 0.659 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.580 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.050 | 6.130 |
Number of reflections | 5146 | |
<I/σ(I)> | 33.87 | 0.43 |
Completeness [%] | 97.0 | 97 |
Redundancy | 4 | 25.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MPD, spermine, magnesium chloride, sodium chloride, sodium cacodylate |