5CJ0
Crystal Structure of Amino Acids 1631-1692 of MYH7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 200.006, 44.355, 74.664 |
Unit cell angles | 90.00, 108.19, 90.00 |
Refinement procedure
Resolution | 49.864 - 2.300 |
R-factor | 0.2173 |
Rwork | 0.216 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ik9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.780 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.310 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.068 | 0.330 |
Number of reflections | 29079 | |
<I/σ(I)> | 40.2 | 3.45 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 14% (w/v) MEPEG 5000, 200 mM glycine, 100 mM bistrispropane pH 7.0. |