5CBH
Structural and Functional Characterization of a Calcium-activated Cation channel from Tsukamurella Paurometabola
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97902 |
Spacegroup name | I 4 |
Unit cell lengths | 116.475, 116.475, 128.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.250 - 3.370 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ahy |
RMSD bond length | 0.014 |
RMSD bond angle | 1.948 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.360 | 3.360 |
Rmerge | 0.740 | |
Number of reflections | 11662 | |
<I/σ(I)> | 3.7 | |
Completeness [%] | 96.2 | 98.3 |
Redundancy | 3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 200, magnesium chloride, cacodylate |