5CAM
Crystal Structure of the Cytoplasmic Domain of the Pseudomonas putida Anti-sigma Factor PupR (SeMet)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.491, 134.691, 34.903 |
Unit cell angles | 90.00, 113.78, 90.00 |
Refinement procedure
Resolution | 67.346 - 2.171 |
R-factor | 0.1818 |
Rwork | 0.178 |
R-free | 0.21650 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.511 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (AutoSol) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 134.700 | 2.290 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 14546 | |
<I/σ(I)> | 18.2 | 1.7 |
Completeness [%] | 97.2 | 82 |
Redundancy | 6.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100mM Bis-Tris pH 6.0, 3M NaCl |