5C33
Crystal Structure of Mouse Ryanodine Receptor 2 SPRY1 Domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.975910, 0.979518, 1.377552 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.246, 64.085, 109.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.371 - 1.210 |
R-factor | 0.1447 |
Rwork | 0.143 |
R-free | 0.17250 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.594 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.371 | 1.250 |
High resolution limit [Å] | 1.210 | 1.210 |
Rmerge | 0.058 | 0.915 |
Number of reflections | 114712 | |
<I/σ(I)> | 17.81 | 2.16 |
Completeness [%] | 99.9 | 98.8 |
Redundancy | 7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.1 M sodium acetate, pH 5.0, 0.6-0.9 M sodium sulfate |