5BWI

Crystallographic structure of a bacterial heparanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-22
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.954
Spacegroup name	P 41 21 2
Unit cell lengths	125.030, 125.030, 125.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.290 - 1.600
R-factor	0.16563
Rwork	0.164
R-free	0.19187
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vnz
RMSD bond length	0.009
RMSD bond angle	1.321
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.290
High resolution limit [Å]	1.600
Number of reflections	129599
<I/σ(I)>	6.9
Completeness [%]	99.4
Redundancy	14.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.8 M potassium sodium tartrate tetrahydrate