5B6F
Crystal structure of the Fab fragment of an anti-Leukotriene C4 monoclonal antibody complexed with LTC4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.600, 173.980, 75.460 |
Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
Resolution | 37.728 - 2.100 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.22240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.941 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.260 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.049 | 0.276 |
Number of reflections | 177824 | |
<I/σ(I)> | 8.7 | 2.4 |
Completeness [%] | 91.1 | 87.3 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 293 | 0.1 microL (0.1M MES-NaOH pH 6.0, 36% (w/v) PEGMME 5000, 0.2M NH4 sulfate, 0.6mM LTC4) + 0.1 microL (15mg/ml Fab, 10mM Na phosphate 7.0, 140mM NaCl) in paraffin oil |