5AVH
The 0.90 angstrom structure (I222) of glucose isomerase crystallized in high-strength agarose hydrogel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-28 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.70000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 93.268, 97.588, 102.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 0.900 |
R-factor | 0.11683 |
Rwork | 0.116 |
R-free | 0.12735 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.122 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.920 |
High resolution limit [Å] | 0.900 | 0.900 |
Rmerge | 0.073 | 0.354 |
Number of reflections | 325304 | |
<I/σ(I)> | 11.6 | 2.38 |
Completeness [%] | 95.1 | 94.3 |
Redundancy | 3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 293 | 0.1M Tris-HCl (pH 7.0), 10% (v/v) MPD, 0.1M Ca acetate |